{"id":20872,"date":"2016-02-04T19:14:38","date_gmt":"2016-02-04T19:14:38","guid":{"rendered":"http:\/\/www.mometrix.com\/academy?page_id=20872"},"modified":"2026-11-02T15:51:27","modified_gmt":"2026-11-02T21:51:27","slug":"mlt-practice-test","status":"publish","type":"page","link":"https:\/\/www.mometrix.com\/academy\/mlt-practice-test\/","title":{"rendered":"MLT Practice Test"},"content":{"rendered":"<p>The Medical Laboratory Technician (MLT) exam is offered by the ASCP Certification Board as a way to obtain MLT certification. Earning your MLT certification demonstrates your dedication, professionalism, and preparation as a medical laboratory technician.<\/p>\n<p>Click \u201cStart Test\u201d above to take a free MLT practice test, and check out our premium-quality MLT test prep resources by clicking the links below!<\/p>\n<div class=\"mx-product-links\">\n<a href=\"https:\/\/www.mometrix.com\/studyguides\/mlt\/\" class=\"mx-guide\">MLT Study Guide<\/a><br \/>\n<a href=\"https:\/\/www.flashcardsecrets.com\/mlt\/\" class=\"mx-flashcards\">MLT Flashcards<\/a>\n<\/div>\n<h2 class=\"pt-page\"><span id=\"Eligibility\" class=\"m-toc-anchor\"><\/span> Eligibility<\/h2>\n<p>To qualify for the MLT exam, you must meet all requirements for one of the three routes below. ASCP discontinued Route 2, which required CLA(ASCP) certification, on January 1, 2023.<\/p>\n<!--- ROUTE 1 --->\r\n\r\n<div class=\"bulb-toggle\">\r\n\t<button class=\"bulb-toggle__button\" type=\"button\" aria-expanded=\"false\">\r\n\t\t<span class=\"bulb-toggle__label\">\r\n\t\t\t<svg class=\"bulb-toggle__icon\" viewBox=\"0 0 24 24\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M6 2h9l5 5v13a2 2 0 0 1-2 2H6a2 2 0 0 1-2-2V4a2 2 0 0 1 2-2zm8 2v4h4l-4-4zm-3.2 13.6 5.7-5.7-1.4-1.4-4.3 4.3-2-2-1.4 1.4 3.4 3.4z\"\/><\/svg>\r\n\t\t\t<span>Route 1<\/span>\r\n\t\t<\/span>\r\n\t\t<svg class=\"bulb-toggle__chevron\" viewBox=\"0 0 20 20\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M5.23 7.21a.75.75 0 0 1 1.06.02L10 11.17l3.71-3.94a.75.75 0 1 1 1.09 1.03l-4.25 4.5a.75.75 0 0 1-1.09 0l-4.25-4.5a.75.75 0 0 1 .02-1.05z\"\/><\/svg>\r\n\t<\/button>\r\n\t<div class=\"bulb-toggle__panel\">\r\n\t\t<div class=\"bulb-toggle__panel-inner\">\r\n\t\t\t<div class=\"bulb-toggle__content\">\r\n\t\t\t\t<p>You need an associate degree or at least 60 semester hours (90 quarter hours) from a regionally or nationally accredited college or university.<\/p>\r\n\t\t\t\t<p>You must also have successfully completed an MLT program accredited by NAACLS or ABHES within the last five years.<\/p>\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>\r\n<\/div>\r\n\r\n<!--- ROUTE 2 \u2014 DISCONTINUED --->\r\n\r\n<div class=\"bulb-toggle\">\r\n\t<button class=\"bulb-toggle__button\" type=\"button\" disabled style=\"background: #f1f1f1; color: #666; border-color: #ddd; cursor: default; box-shadow: none; pointer-events: none;\">\r\n\t\t<span class=\"bulb-toggle__label\">\r\n\t\t\t<svg class=\"bulb-toggle__icon\" viewBox=\"0 0 24 24\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M6 2h9l5 5v13a2 2 0 0 1-2 2H6a2 2 0 0 1-2-2V4a2 2 0 0 1 2-2zm8 2v4h4l-4-4zm-3.2 13.6 5.7-5.7-1.4-1.4-4.3 4.3-2-2-1.4 1.4 3.4 3.4z\"\/><\/svg>\r\n\t\t\t<span style=\"color: #666;\">Route 2 \u2014 Discontinued<\/span>\r\n\t\t<\/span>\r\n\t<\/button>\r\n<\/div>\r\n\r\n<!--- ROUTE 3 --->\r\n\r\n<div class=\"bulb-toggle\">\r\n\t<button class=\"bulb-toggle__button\" type=\"button\" aria-expanded=\"false\">\r\n\t\t<span class=\"bulb-toggle__label\">\r\n\t\t\t<svg class=\"bulb-toggle__icon\" viewBox=\"0 0 24 24\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M6 2h9l5 5v13a2 2 0 0 1-2 2H6a2 2 0 0 1-2-2V4a2 2 0 0 1 2-2zm8 2v4h4l-4-4zm-3.2 13.6 5.7-5.7-1.4-1.4-4.3 4.3-2-2-1.4 1.4 3.4 3.4z\"\/><\/svg>\r\n\t\t\t<span>Route 3<\/span>\r\n\t\t<\/span>\r\n\t\t<svg class=\"bulb-toggle__chevron\" viewBox=\"0 0 20 20\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M5.23 7.21a.75.75 0 0 1 1.06.02L10 11.17l3.71-3.94a.75.75 0 1 1 1.09 1.03l-4.25 4.5a.75.75 0 0 1-1.09 0l-4.25-4.5a.75.75 0 0 1 .02-1.05z\"\/><\/svg>\r\n\t<\/button>\r\n\t<div class=\"bulb-toggle__panel\">\r\n\t\t<div class=\"bulb-toggle__panel-inner\">\r\n\t\t\t<div class=\"bulb-toggle__content\">\r\n\t\t\t\t<p>You need an associate degree or at least 60 semester hours (90 quarter hours) from a regionally or nationally accredited college or university. Your coursework must include at least 6 semester hours (9 quarter hours) each in biology and chemistry. These science credits may be earned as part of your degree or separately.<\/p>\r\n\t\t\t\t<p>You must also have successfully completed a 50-week U.S. military medical laboratory training course within the last ten years.<\/p>\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>\r\n<\/div>\r\n\r\n<!--- ROUTE 4 --->\r\n\r\n<div class=\"bulb-toggle\">\r\n\t<button class=\"bulb-toggle__button\" type=\"button\" aria-expanded=\"false\">\r\n\t\t<span class=\"bulb-toggle__label\">\r\n\t\t\t<svg class=\"bulb-toggle__icon\" viewBox=\"0 0 24 24\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M6 2h9l5 5v13a2 2 0 0 1-2 2H6a2 2 0 0 1-2-2V4a2 2 0 0 1 2-2zm8 2v4h4l-4-4zm-3.2 13.6 5.7-5.7-1.4-1.4-4.3 4.3-2-2-1.4 1.4 3.4 3.4z\"\/><\/svg>\r\n\t\t\t<span>Route 4<\/span>\r\n\t\t<\/span>\r\n\t\t<svg class=\"bulb-toggle__chevron\" viewBox=\"0 0 20 20\" aria-hidden=\"true\"><path fill=\"currentColor\" d=\"M5.23 7.21a.75.75 0 0 1 1.06.02L10 11.17l3.71-3.94a.75.75 0 1 1 1.09 1.03l-4.25 4.5a.75.75 0 0 1-1.09 0l-4.25-4.5a.75.75 0 0 1 .02-1.05z\"\/><\/svg>\r\n\t<\/button>\r\n\t<div class=\"bulb-toggle__panel\">\r\n\t\t<div class=\"bulb-toggle__panel-inner\">\r\n\t\t\t<div class=\"bulb-toggle__content\">\r\n\t\t\t\t<p>You need an associate degree or at least 60 semester hours (90 quarter hours) from a regionally or nationally accredited college or university. Your coursework must include at least 6 semester hours (9 quarter hours) each in biology and chemistry. These science credits may be earned as part of your degree or separately.<\/p>\r\n\t\t\t\t<p>You must also have three years of acceptable full-time clinical laboratory experience within the last six years. Your experience must cover blood banking, chemistry, hematology, microbiology, immunology, and urinalysis\/body fluids. You need experience in every area, but not three full years in each.<\/p>\r\n\t\t\t\t<p>The laboratory must hold a qualifying CMS CLIA or DoD CLIP certificate, JCI accreditation, or ISO 15189 accreditation.<\/p>\r\n\t\t\t\t<p>One year of the experience requirement may be replaced by successful completion, within the last five years, of a NAACLS-accredited MLS program, a NAACLS- or ABHES-accredited MLT program, or a foreign medical laboratory science clinical training program.<\/p>\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>\r\n<\/div>\n<h2 class=\"pt-page\"><span id=\"MLT_Exam_Outline\" class=\"m-toc-anchor\"><\/span>MLT Exam Outline<\/h2>\n<p>The MLT exam contains 100 multiple-choice questions and has a time limit of 2.5 hours.<\/p>\n<p><a href=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2023\/11\/ASCP-MLT-Exam-Outline-01.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208120\" role=\"img\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2023\/11\/ASCP-MLT-Exam-Outline-01.svg\" alt=\"exam outline for the ASCP MLT exam\" width=\"367\" height=\"437\" \/><\/a><\/p>\n<p>This exam is a computer-adaptive test (CAT), so depending on your performance, the difficulty of your test will increase or decrease as you go on.<\/p>\n<p>Here\u2019s a look at how it works:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-138529\" role=\"img\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2022\/08\/Computer-Adaptive-Flowchart.svg\" alt=\"A flowchart that shows the adaptive nature of the exam\" width=\"467\" height=\"746\" \/><\/p>\n<p>The first question is judged to be of medium difficulty, and depending on your performance, the next question may be easier or harder. If you do well on the first question, the second question will be harder; conversely, if you do poorly on the first question, the second question will be easier.<\/p>\n<h3><span id=\"1_Blood_Banking_(15%2520%25)\" class=\"m-toc-anchor\"><\/span>1. Blood Banking (15%-20%)<\/h3>\n<p>These questions assess your knowledge of blood products, group systems, immunology, physiology and pathophysiology, serologic and molecular testing, and transfusion practice.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Blood Products<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Donors<\/li>\n<li>Processing<\/li>\n<li>Storage<\/li>\n<li>Blood components<\/li>\n<li>Blood component quality control<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Blood Group Systems<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Genetics<\/li>\n<li>Antigens\/Biochemistry<\/li>\n<li>Role of blood groups in transfusion<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Blood Group Immunology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Immune response<\/li>\n<li>Immunoglobulins<\/li>\n<li>Antigen-antibody interactions<\/li>\n<li>Complement<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>IV. Physiology and Pathophysiology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Blood physiology<\/li>\n<li>Coagulation and hemostasis<\/li>\n<li>Fetus and newborn hemolytic disease<\/li>\n<li>Anemias<\/li>\n<li>Transplantation<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>V. Serologic and Molecular Testing<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Routine tests<\/li>\n<li>Reagents<\/li>\n<li>Application of reagents and special tests<\/li>\n<li>Platelet\/Leukocyte testing<\/li>\n<li>Quality assurance<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">VI. Transfusion Practice<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Indications for transfusion<\/li>\n<li>Component therapy<\/li>\n<li>Adverse transfusion effects<\/li>\n<li>Apheresis and extracorporeal circulation<\/li>\n<li>Blood administration<\/li>\n<li>Patient blood management<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"2_Urinalysis_and_Body_Fluids_(5%2510%25)\" class=\"m-toc-anchor\"><\/span>2. Urinalysis and Body Fluids (5%-10%)<\/h3>\n<p>These questions assess your knowledge of physical and chemical testing, microscopic analysis, physiology, and disease states.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Urinalysis<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Physical<\/li>\n<li>Microscopic<\/li>\n<li>Chemical<\/li>\n<li>Renal physiology<\/li>\n<li>Disease states<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">II. Body Fluids<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Physical<\/li>\n<li>Microscopic<\/li>\n<li>Chemical<\/li>\n<li>Physiology<\/li>\n<li>Disease states<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"3_Chemistry_(20%2525%25)\" class=\"m-toc-anchor\"><\/span>3. Chemistry (20%-25%)<\/h3>\n<p>These questions assess your knowledge of carbohydrates, lipids, heme derivatives, enzymes, proteins, and other nitrogen-containing compounds. Additional topics include acid-base determinations, electrolytes, endocrinology, vitamins and nutrition, therapeutic drug monitoring, and toxicology.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. General Chemistry<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Carbohydrates<\/li>\n<li>Heme derivatives<\/li>\n<li>Lipids<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Proteins and Enzymes<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Proteins and other nitrogen-containing compounds<\/li>\n<li>Enzymes<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Acid-base, Blood Gases, and Electrolytes<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Acid-base determinations<\/li>\n<li>Electrolytes<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">IV. Special Chemistry<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Therapeutic drug monitoring<\/li>\n<li>Endocrinology<\/li>\n<li>Toxicology<\/li>\n<li>Vitamins and nutrition<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"4_Hematology_(20%2525%25)\" class=\"m-toc-anchor\"><\/span>4. Hematology (20%-25%)<\/h3>\n<p>These questions assess your knowledge of physiology, disease states, laboratory testing, and hemostasis (including physiology, disease states, and laboratory determinations).<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Hematology Physiology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Function<\/li>\n<li>Production<\/li>\n<li>Destruction<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Hematology Disease States<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Leukocytes (WHO classification)<\/li>\n<li>Erythrocytes<\/li>\n<li>Platelets<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Hematology Laboratory Testing<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Hemoglobin<\/li>\n<li>Cell counts<\/li>\n<li>Flow cytometry immunophenotyping<\/li>\n<li>Differentials and morphology evaluation<\/li>\n<li>Molecular and cytogenetic testing<\/li>\n<li>Hematocrit<\/li>\n<li>Indices<\/li>\n<li>Special stains<\/li>\n<li>Hemolytic indicators<\/li>\n<li>Other studies<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">IV. Hemostasis<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Disease states<\/li>\n<li>Physiology<\/li>\n<li>Laboratory determinations<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"5_Immunology_(5%2510%25)\" class=\"m-toc-anchor\"><\/span>5. Immunology (5%-10%)<\/h3>\n<p>These questions assess your knowledge of immunology principles, immune system diseases, transplantation, infectious disease serology, serologic procedures, and test results.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Principles of Immunology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Immunoglobulins<\/li>\n<li>Immune system physiology<\/li>\n<li>Antigen-antibody interactions<\/li>\n<li>Complement<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Diseases of the Immune System<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Hypersensitivity<\/li>\n<li>Immunodeficiency<\/li>\n<li>Autoimmunity<\/li>\n<li>Immunoproliferative diseases<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Transplantation<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>HLA typing<\/li>\n<li>Graft-vs-host disease<\/li>\n<li>Tumor immunology<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>IV. Infectious Disease Serology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Stages of infection of <em>Treponema pallidum<\/em> and <em>Borrelia burgdorferi<\/em><\/li>\n<li>Clinical significance and epidemiology of viral pathogens<\/li>\n<li>Tuberculosis infection<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>V. Serologic Procedures<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Rheumatoid factor<\/li>\n<li>Cytokine testing<\/li>\n<li>ANA<\/li>\n<li>Immunofluorescence<\/li>\n<li>Thyroid antibodies<\/li>\n<li>Nontreponemal syphilis testing<\/li>\n<li>Labeled immunoassays<\/li>\n<li>Treponemal syphilis testing<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">VI. Test Results<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Interpretation<\/li>\n<li>Confirmatory testing<\/li>\n<li>Disease state correlation<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"6_Microbiology_(15%2520%25)\" class=\"m-toc-anchor\"><\/span>6. Microbiology (15%-20%)<\/h3>\n<p>These questions assess your knowledge of pre-analytic procedures, analytic procedures for bacteriology, and analytic procedures for mycobacteriology, virology, parasitology, and mycology, and post-analytic procedures.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Preanalytic Procedures<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Specimen collection and transport<\/li>\n<li>Specimen processing<\/li>\n<li>Stain procedures, principles, and interpretations<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Analytic Procedures for Bacteriology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Cerebrospinal fluid<\/li>\n<li>Upper respiratory<\/li>\n<li>Lower respiratory<\/li>\n<li>Blood and bone marrow<\/li>\n<li>Body fluids from normally sterile sites<\/li>\n<li>Genital tract<\/li>\n<li>Antimicrobial susceptibility testing and antibiotic resistance<\/li>\n<li>Gastrointestinal<\/li>\n<li>MRSA\/MSSA, VRE, ESBL\/CRE screening<\/li>\n<li>Skin, soft tissue, and bone<\/li>\n<li>Urine<\/li>\n<li>BSL-3 pathogens and select agents (bioterrorism)<\/li>\n<li>Identification methods<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Analytic Procedures for Mycobacteriology, Virology, Parasitology, and Mycology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Mycobacteriology and <em>Nocardia<\/em> spp.<\/li>\n<li>Virology<\/li>\n<li>Parasitology<\/li>\n<li>Mycology<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">IV. Postanalytic Procedures<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Documentation practices<\/li>\n<li>Critical and urgent value reporting<\/li>\n<li>Result review and autoverification<\/li>\n<li>Issuing corrected reports<\/li>\n<li>Reporting to infection control\/prevention and public health<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"7_Laboratory_Operations_(5%2510%25)\" class=\"m-toc-anchor\"><\/span>7. Laboratory Operations (5%-10%)<\/h3>\n<p>These questions cover quality assessment and troubleshooting, safety, laboratory mathematics, and manual and automated methodology and instrumentation.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Quality Assessment\/Troubleshooting<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Preanalytical, analytical, and postanalytical<\/li>\n<li>Point-of-care testing<\/li>\n<li>Regulation<\/li>\n<li>Quality control<\/li>\n<li>Compliance<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Safety<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Safety programs and practices<\/li>\n<li>Emergency procedures<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Laboratory Mathematics<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Standard curves<\/li>\n<li>Sensitivity, specificity, and predictive value<\/li>\n<li>Molarity and normality<\/li>\n<li>Mean, median, mode and confidence intervals<\/li>\n<li>Concentration, volume, and dilution<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>IV. Manual and Automated Methodology and Instrumentation<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Basic laboratory equipment<\/li>\n<li>Osmometry<\/li>\n<li>Nephelometry<\/li>\n<li>Electrophoresis<\/li>\n<li>Flow cytometry<\/li>\n<li>Spectrophotometry and photometry<\/li>\n<li>Chromatography<\/li>\n<li>Fluorometry<\/li>\n<li>Mass spectrometry<\/li>\n<li>Electrochemistry<\/li>\n<li>Molecular methods<\/li>\n<li>Hematology instrumentation<\/li>\n<li>Automated microbiology processors<\/li>\n<\/ul>\n<\/details>\n\n\t\t\t<div class=\"sg-box2\">\n\t\t\t\t<div class=\"sg-box2-text\">\n\t\t\t\t\t<p class=\"sg-box2-title\">Check Out Mometrix&#039;s MLT Study Guide!<\/p>\n\t\t\t\t\t<p>Get practice questions, video tutorials, and detailed study lessons.<\/p>\n\t\t\t\t\t<p><a class=\"sg-box2-button\" href=\"https:\/\/www.mometrix.com\/studyguides\/mlt\/\" target=\"_blank\" rel=\"noopener\">Get Your Study Guide<span aria-hidden=\"true\">&nbsp;&rarr;<\/span><\/a><\/p>\n\t\t\t\t<\/div>\n\t\t\t\t<p><a href=\"https:\/\/www.mometrix.com\/studyguides\/mlt\/\"><img loading=\"lazy\" decoding=\"async\" width=\"184\" height=\"238\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2021\/06\/mlt.jpg\" alt=\" Study Guide\"><\/a><\/p>\n\t\t\t<\/div>\n<h2 class=\"pt-page\"><span id=\"How_to_Pass\" class=\"m-toc-anchor\"><\/span>How to Pass<\/h2>\n<p>Think you aren\u2019t a good test-taker? Maybe on a study-time crunch? Or just don\u2019t know how to begin studying? Mometrix has designed a new Study Secrets course to help every student, no matter what study scenario you are in. Here\u2019s what you\u2019ll find in the Study Secrets Course:<\/p>\n<ul>\n<li>Techniques to Conquer Procrastination<\/li>\n<li>Steps to building a Study Plan custom to your learning style<\/li>\n<li>7 Effective Note-Taking Methods<\/li>\n<li>Test-Taking Tips<\/li>\n<li>Memory Techniques and Mnemonics<\/li>\n<li>And much more!<\/li>\n<\/ul>\n<p>Everyone learns differently, so we\u2019ve tailored our Study Secrets Course to ensure every learner has what they need to prepare for their upcoming exam or semester. Click below to check it out!<\/p>\n<div class=\"buttonlinks\"><a href=\"https:\/\/www.mometrix.com\/university\/studysecrets\/\" class=\"class_names\" style=\"color:black\" onclick=\"_paq.push([&quot;trackEvent&quot;,&quot;Course Button&quot;,&quot;Course Click&quot;,&quot;Study Course Click\\u0027&quot;]);\">Study Secrets Online Course<\/a><\/div>\n<h2 class=\"pt-page\"><span id=\"Registration\" class=\"m-toc-anchor\"><\/span>Registration<\/h2>\n<p>To register for the MLT exam, start by applying online through your ASCP account. You'll need to submit the documentation required for your eligibility route and pay the nonrefundable $235 application fee. Arrange for any required official transcripts to be sent to ASCP.<\/p>\n<p>After ASCP approves your eligibility, you\u2019ll receive an email directing you to your Admission Notification. Use those instructions to schedule your exam at an approved Pearson testing center within your eligibility period.<\/p>\n<h2 class=\"pt-page\"><span id=\"Test_Day\" class=\"m-toc-anchor\"><\/span>Test Day<\/h2>\n<p>You should arrive at the testing center 30 minutes before your exam is scheduled to begin. Bring an accepted, unexpired ID containing your photograph and signature, and make sure that your first and last names match those on your Admission Notification. During check-in, Pearson will collect your signature, photograph, and palm-vein image.<\/p>\n<p>In general, avoid bringing any personal items with you, such as a cell phone, pencils, bags, hats, etc.<\/p>\n<h2 class=\"pt-page\"><span id=\"How_the_Test_is_Scored\" class=\"m-toc-anchor\"><\/span>How the Test is Scored<\/h2>\n<p>You can view your preliminary pass\/fail result when you finish the exam. ASCP normally emails instructions for accessing your official score report within four business days, provided your required official transcripts have been received and processed.<\/p>\n<p>Keep in mind that you are not credentialed until your passing score report is available.<\/p>\n<p>The score you need to pass the exam is 400.<\/p>\n\n\t\t\t<div class=\"sg-box2 fc-box\">\n\t\t\t\t<div class=\"sg-box2-text\">\n\t\t\t\t\t<p class=\"sg-box2-title\">Check Out Mometrix&#039;s MLT Flashcards!<\/p>\n\t\t\t\t\t<p>Get complex  subjects broken down into easily understandable concepts.<\/p>\n\t\t\t\t\t<p><a class=\"fc-box-button\" href=\"https:\/\/www.flashcardsecrets.com\/mlt\/\" target=\"_blank\" rel=\"noopener\">Get Your Flashcards<span aria-hidden=\"true\">&nbsp;&rarr;<\/span><\/a><\/p>\n\t\t\t\t<\/div>\n\t\t\t\t<p><a href=\"https:\/\/www.flashcardsecrets.com\/mlt\/\"><img loading=\"lazy\" decoding=\"async\" width=\"238\" height=\"184\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2021\/06\/mltflash.jpg\" alt=\" Flashcards\"><\/a><\/p>\n\t\t\t<\/div>\n<h2 class=\"pt-page\"><span id=\"Study_Tips\" class=\"m-toc-anchor\"><\/span>Study Tips<\/h2>\n<h3>Start with an MLT Practice Test<\/h3>\n<p>Before creating your study schedule, take an MLT practice test to establish a starting point. You don't need to wait until you have reviewed every blood group, chemistry analyte, blood cell abnormality, or microorganism. At this stage, the goal is to identify where your preparation time will have the greatest effect.<\/p>\n<p>Review your results across the seven content areas:<\/p>\n<ul>\n<li style=\"margin-bottom: 10px\"><strong style=\"font-weight: 600\">Blood Banking:<\/strong> Can you apply blood group systems, antibody identification, compatibility testing, blood-component selection, transfusion practices, and reactions?<\/li>\n<li style=\"margin-bottom: 10px\"><strong style=\"font-weight: 600\">Urinalysis and Other Body Fluids:<\/strong> Can you connect physical, chemical, and microscopic findings with physiology and disease states?<\/li>\n<li style=\"margin-bottom: 10px\"><strong style=\"font-weight: 600\">Chemistry:<\/strong> Can you interpret results involving carbohydrates, electrolytes, enzymes, proteins, acid-base balance, endocrinology, therapeutic drugs, and toxicology?<\/li>\n<li style=\"margin-bottom: 10px\"><strong style=\"font-weight: 600\">Hematology:<\/strong> Can you recognize normal and abnormal blood-cell findings, correlate results with disease states, and interpret coagulation testing?<\/li>\n<li style=\"margin-bottom: 10px\"><strong style=\"font-weight: 600\">Immunology:<\/strong> Do you understand immune-system principles, serologic procedures, infectious-disease testing, and how to interpret test results?<\/li>\n<li style=\"margin-bottom: 10px\"><strong style=\"font-weight: 600\">Microbiology:<\/strong> Can you use specimen information, morphology, staining, culture characteristics, and biochemical or other testing to identify microorganisms?<\/li>\n<li><strong style=\"font-weight: 600\">Laboratory Operations:<\/strong> Can you apply quality-control principles, troubleshoot instruments and methods, perform laboratory calculations, and follow safety requirements?<\/li>\n<\/ul>\n<p>Identifying those specific weaknesses will show you where to devote more attention during your study time.<\/p>\n<h3><span id=\"Review_Difficult_Questions\" class=\"m-toc-anchor\"><\/span>Review Difficult Questions<\/h3>\n<p>After each practice session, review every question you answered incorrectly AND every question you guessed on or answered with low confidence.<\/p>\n<p>For each difficult question, determine which of the following caused the problem:<\/p>\n<ul>\n<li>You knew the laboratory value but didn't understand what could cause it to increase or decrease.<\/li>\n<li>You recognized an abnormal result but couldn't connect it to the likely disease process.<\/li>\n<li>You confused two organisms or lab methods with similar characteristics.<\/li>\n<li>You selected the correct test but interpreted the result incorrectly.<\/li>\n<li>You overlooked a specimen-collection problem that could affect the result.<\/li>\n<li>You made an error in a laboratory calculation.<\/li>\n<li>You knew a testing procedure but didn't recognize the appropriate quality-control step.<\/li>\n<li>You focused on one abnormal result instead of considering the patient's results as a pattern.<\/li>\n<\/ul>\n<p>Then, outline a specific plan for improvement based on what you discovered. In other words, don't just say, \u201cI need to study hematology.\u201d Note that you need to practice distinguishing microcytic anemias using RBC indices, morphology, and iron-study results.<\/p>\n<p>Similarly, replace \u201cstudy microbiology\u201d with something specific, such as distinguishing gram-negative enteric organisms using colony characteristics and biochemical reactions.<\/p>\n<h3><span id=\"Utilize_Answer_Explanations\" class=\"m-toc-anchor\"><\/span>Utilize Answer Explanations<\/h3>\n<p>Answer explanations are an underutilized studying resource. To take full advantage of them, read the explanation for every question you found difficult, including questions you answered correctly.<\/p>\n<p>For each one, make sure you can confidently explain:<\/p>\n<ol>\n<li>Why the correct answer is correct<\/li>\n<li>Why each incorrect answer is less appropriate<\/li>\n<li>What lab principle or disease process is being tested<\/li>\n<li>Which patient result or laboratory finding controls the answer<\/li>\n<li>What the laboratory professional should do next<\/li>\n<li>How the same principle could appear with a different specimen, result pattern, or patient condition<\/li>\n<\/ol>\n<p>For patient-result questions, don't stop after identifying the abnormal value. Determine how the results relate to one another and what physiological or disease process could produce that pattern.<\/p>\n<p>For procedural questions, reconstruct the workflow. Identify the specimen requirements, testing method, expected result, quality-control requirements, and what should happen if the result or control is unacceptable.<\/p>\n<h3><span id=\"Study_Laboratory_Results_as_Patterns\" class=\"m-toc-anchor\"><\/span>Study Laboratory Results as Patterns<\/h3>\n<p>The MLT exam doesn't only test whether you know normal ranges or isolated facts. Practice connecting laboratory findings to one another and to the patient's condition.<\/p>\n<p>Use this process:<\/p>\n<p class=\"longmath\" style=\"margin-left: 20px; font-weight: 600\">Patient Information \u2192 Test Results \u2192 Pattern \u2192 Likely Cause \u2192 Next Laboratory Step<\/p>\n<p>For example, don't memorize individual RBC indices without connecting them. Use the hemoglobin, hematocrit, MCV, MCHC, RDW, morphology, and other relevant results together to determine what type of anemia the pattern suggests.<\/p>\n<p>Apply the same approach in chemistry. Instead of memorizing individual liver, renal, or endocrine tests, practice recognizing how related analytes change together and what those relationships suggest.<\/p>\n<h2 class=\"pt-page\"><span id=\"FAQs\" class=\"m-toc-anchor\"><\/span>FAQs<\/h2>\n<div class=\"faq-list\">\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n\t\t<span class=\"letter\" aria-hidden=\"true\">Q<\/span><\/p>\n<h3 class=\"faq-question\">How many questions are on the MLT exam?<\/h3>\n<\/p><\/div>\n<div class=\"a_item\">\n\t\t<span class=\"letter text_bold\" aria-hidden=\"true\">A<\/span><\/p>\n<p>There are 100 multiple-choice questions on the exam.<\/p>\n<\/p><\/div>\n<\/div>\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n\t\t<span class=\"letter\" aria-hidden=\"true\">Q<\/span><\/p>\n<h3 class=\"faq-question\">How long is the MLT exam?<\/h3>\n<\/p><\/div>\n<div class=\"a_item\">\n\t\t<span class=\"letter text_bold\" aria-hidden=\"true\">A<\/span><\/p>\n<p>The time limit for the exam is 2.5 hours.<\/p>\n<\/p><\/div>\n<\/div>\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n\t\t<span class=\"letter\" aria-hidden=\"true\">Q<\/span><\/p>\n<h3 class=\"faq-question\">What is the passing score for the MLT exam?<\/h3>\n<\/p><\/div>\n<div class=\"a_item\">\n\t\t<span class=\"letter text_bold\" aria-hidden=\"true\">A<\/span><\/p>\n<p>You&#8217;ll need a final score of at least 400 to pass.<\/p>\n<\/p><\/div>\n<\/div>\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n\t\t<span class=\"letter\" aria-hidden=\"true\">Q<\/span><\/p>\n<h3 class=\"faq-question\">How much is the MLT exam?<\/h3>\n<\/p><\/div>\n<div class=\"a_item\">\n\t\t<span class=\"letter text_bold\" aria-hidden=\"true\">A<\/span><\/p>\n<p>The cost of the exam is $235.<\/p>\n<\/p><\/div>\n<\/div>\n<\/div>\n\n<p><div itemscope=\"\" itemprop=\"author\" itemtype=\"https:\/\/schema.org\/Person\" class=\"author-bio\" id=\"author-bio-kate-nockunas\"><img loading=\"lazy\" decoding=\"async\" itemprop=\"image\" class=\"author-image\" height=\"127\" width=\"127\" alt=\"Headshot of Kate Nockunas.\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2022\/06\/tinywow_tinywow_Kate-Nockunas-Cropped-min_24640345_24640389.webp\"><div class=\"author-text\"><p class=\"author-name\">By <span itemprop=\"name\">Kate Nockunas<\/span><\/p><div class=\"author-desc\"><p>Kate has been Mometrix\u2019s Nursing and Medical Editor for over four years. She has a bachelor\u2019s degree in Communications from Vanderbilt University, a bachelor\u2019s degree in Nursing from Marymount University, and a master\u2019s degree in Education from Johns Hopkins. Kate is a critical care registered nurse who still works occasionally in the ICU and PACU.<\/p><\/div><\/div><\/div><div itemscope=\"\" itemprop=\"editor\" itemtype=\"https:\/\/schema.org\/Person\" class=\"author-bio\" id=\"editor-bio-aaron-lanni\"><img loading=\"lazy\" decoding=\"async\" itemprop=\"image\" class=\"author-image\" height=\"127\" width=\"127\" alt=\"Aaron Lanni profile pic\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2024\/12\/image_2024-12-11_124906662.png\"><div class=\"author-text\"><p class=\"author-name\">Edited by <span itemprop=\"name\">Aaron Lanni<\/span><\/p><div class=\"author-desc\"><p>Aaron is the content manager and lead editor for Mometrix Academy. He regularly produces, updates, proofreads, and edits content to ensure it meets Mometrix\u2019s quality and accessibility standards.<\/p><\/div><p class=\"alignright author-desc author-link\"><a class=\"ylist\" href=\"https:\/\/www.mometrix.com\/academy\/editorial-guidelines\/\">Learn more about our editorial guidelines<\/a> \u2192<\/p><\/div><\/div><\/p>\n<div class=\"home-buttons\">\n<p><a href=\"https:\/\/www.mometrix.com\/academy\/\">Mometrix Academy - Home<\/a><\/p>\n<\/div>\n<p class=\"disclaimer\">Mometrix Test Preparation is not affiliated with or endorsed by any official testing organization. All organizational and test names are trademarks of their respective owners.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Medical Laboratory Technician (MLT) exam is offered by the ASCP Certification Board as a way to obtain MLT certification. Earning your MLT certification demonstrates your dedication, professionalism, and preparation as a medical laboratory technician. Click \u201cStart Test\u201d above to take a free MLT practice test, and check out our premium-quality MLT test prep resources &#8230; <a title=\"MLT Practice Test\" class=\"read-more\" href=\"https:\/\/www.mometrix.com\/academy\/mlt-practice-test\/\" aria-label=\"Read more about MLT Practice Test\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":202076,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":{"0":"post-20872","1":"page","2":"type-page","3":"status-publish","4":"has-post-thumbnail","6":"page_category-long-math","7":"page_category-medical-technology","8":"page_type-exam-overview","9":"page_domain-ascp-mlt"},"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 5.0.5 - aioseo.com -->\n\t<meta name=\"description\" content=\"Take a free MLT practice test for medical laboratory technician certification. 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