{"id":158126,"date":"2022-12-20T11:33:30","date_gmt":"2022-12-20T17:33:30","guid":{"rendered":"https:\/\/www.mometrix.com\/academy\/?page_id=158126"},"modified":"2026-05-14T14:26:50","modified_gmt":"2026-05-14T19:26:50","slug":"medical-laboratory-science-practice-test","status":"publish","type":"page","link":"https:\/\/www.mometrix.com\/academy\/medical-laboratory-science-practice-test\/","title":{"rendered":"ASCP Medical Laboratory Science Exam"},"content":{"rendered":"<p>The American Society for Clinical Pathology (ASCP) administers the Medical Laboratory Science (MLS) exam for those wishing to obtain a credential as a medical laboratory scientist.<\/p>\n<p>Click \u201cStart Test\u201d above to take a free ASCP Medical Laboratory Science practice test, and check out our premium-quality ASCP Medical Laboratory Science test prep resources by clicking the links below!<\/p>\n<div class=\"buttonlinks\">\n<a href=\"https:\/\/www.mometrix.com\/studyguides\/mls\/\" class=\"class_names\" style=\"color:black;\" onclick=\"_paq.push(['trackEvent', 'Guide Button', 'Guide Click', 'MLS Guide Click']);\">Medical Laboratory Science Study Guide<\/a><br \/>\n<a href=\"https:\/\/www.flashcardsecrets.com\/mls\/\" class=\"class_names\" style=\"color:black;\" onclick=\"_paq.push(['trackEvent', 'Flashcard Button', 'Flashcard Button Click', 'MLS Flashcard Click']);\">Medical Laboratory Science Flashcards<\/a>\n<\/div>\n<h2 class=\"pt-page\"><span id=\"ASCP_MLS_Exam_Eligibility\" class=\"m-toc-anchor\"><\/span>ASCP MLS Exam Eligibility<\/h2>\n<p>There are five routes to meeting eligibility requirements for this exam. It doesn&#8217;t matter which route you choose, but you must meet ALL of the eligibility requirements for a particular route. It is not possible to \u201cmix and match\u201d eligibility requirements.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.15em;\">Route 1<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>A baccalaureate degree from a regionally accredited college or university<\/li>\n<li>Successful completion of a National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) accredited Medical Laboratory Scientist program within the last five years<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>Route 2<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>A valid Medical Laboratory Technician (MLT) American Society for Clinical Pathology (ASCP) certification<\/li>\n<li>A baccalaureate degree from a regionally accredited college with one semester in microbiology, 16 semester hours in chemistry, and one semester in organic or biochemistry<\/li>\n<li>Two years of full-time clinical experience* in blood banking, chemistry, hematology, microbiology, immunology, and urinalysis\/body fluids in a laboratory within the last five years<\/li>\n<\/ul>\n<p style=\"font-size: small;\"><em>*Completion of a NAACLS-accredited MLS program, NAACLS or Accrediting Bureau of Health Education Schools (ABHES)-accredited MLT program, or a foreign medical laboratory science clinical training program within the last five years can be used for one year of clinical experience and one semester of organic or biochemistry.<\/em><\/p>\n<\/details>\n<details class=\"togglelist\">\n<summary>Route 3<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>A baccalaureate degree from a regionally accredited college with one semester in microbiology, 16 semester hours in chemistry, and one semester in organic or biochemistry<\/li>\n<li>Five years of full-time clinical experience* in blood banking, chemistry, hematology, microbiology, immunology, and urinalysis\/body fluids in a laboratory within the last ten years<\/li>\n<\/ul>\n<p style=\"font-size: small;\"><em>*Completion of a NAACLS-accredited MLS program, NAACLS or Accrediting Bureau of Health Education Schools (ABHES)-accredited MLT program, or a foreign medical laboratory science clinical training program within the last five years can be used for one year of clinical experience and one semester of organic or biochemistry.<\/em><\/p>\n<\/details>\n<details class=\"togglelist\">\n<summary>Route 4<\/summary>\n<p style=\"margin-top: 0.5em;\">This eligibility method is generally for individuals with foreign education and experience.<\/p>\n<ul style=\"list-style-type: circle;\">\n<li>A valid Medical Technologist (MT)\/MLS (ASCP) certification<\/li>\n<li>A transcript evaluation verifying equivalency to a US baccalaureate degree from a regionally accredited college or university<\/li>\n<li>Five years of full-time clinical experience in blood banking, chemistry, hematology, microbiology, immunology, and urinalysis\/body fluids in a laboratory within the last ten years<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1em;\">Route 5<\/summary>\n<p style=\"margin-top: 0.5em;\">This eligibility method is for individuals with military experience.<\/p>\n<ul style=\"list-style-type: circle;\">\n<li>Successful completion of a 50-week US military medical laboratory training course within the last ten years<\/li>\n<li>A baccalaureate degree from a regionally accredited college or university with 16 semester hours in biology, including one semester in microbiology and 16 semester hours in chemistry<\/li>\n<li>One year of full-time clinical experience in blood banking, chemistry, hematology, microbiology, immunology, and urinalysis\/body fluids in a laboratory within the last ten years<\/li>\n<\/ul>\n<\/details>\n<h2 class=\"pt-page\"><span id=\"ASCP_MLS_Exam_Outline\" class=\"m-toc-anchor\"><\/span>ASCP MLS Exam Outline<\/h2>\n<p>The Medical Laboratory Scientist exam is a computer-adaptive test (CAT) consisting of 100 multiple-choice questions, and you will be given a 2.5-hour time limit.<\/p>\n<p>The questions on the exam are split into two types: theoretical and procedural.<\/p>\n<ul>\n<li><strong>Theoretical:<\/strong> These questions measure your ability to apply knowledge effectively, calculate results accurately, and correlate patient results to disease states.<\/li>\n<li><strong>Procedural:<\/strong> These questions measure your skills in performing laboratory techniques and following quality assurance protocols.<\/li>\n<\/ul>\n<p>There are seven content areas on the exam:<\/p>\n<h3><span id=\"1_Blood_Banking_(17%2522%25)\" class=\"m-toc-anchor\"><\/span>1. Blood Banking (17%-22%)<\/h3>\n<p>These questions assess your knowledge of blood products, group systems, immunology, physiology and pathophysiology, serologic and molecular testing, and transfusion practice.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Blood Products<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Donors<\/li>\n<li>Processing<\/li>\n<li>Storage<\/li>\n<li>Blood components<\/li>\n<li>Blood component quality control<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Blood Group Systems<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Genetics<\/li>\n<li>Antigens\/Biochemistry<\/li>\n<li>Role of blood groups in transfusion<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Blood Group Immunology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Immune response<\/li>\n<li>Immunoglobulins<\/li>\n<li>Antigen-antibody interactions<\/li>\n<li>Complement<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>IV. Physiology and Pathophysiology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Blood physiology<\/li>\n<li>Coagulation and hemostasis<\/li>\n<li>Fetus and newborn hemolytic disease<\/li>\n<li>Anemias<\/li>\n<li>Transplantation<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>V. Serologic and Molecular Testing<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Routine tests<\/li>\n<li>Reagents<\/li>\n<li>Application of reagents and special tests<\/li>\n<li>Platelet\/Leukocyte testing<\/li>\n<li>Quality assurance<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1.5em;\">VI. Transfusion Practice<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Indications for transfusion<\/li>\n<li>Component therapy<\/li>\n<li>Adverse transfusion effects<\/li>\n<li>Apheresis and extracorporeal circulation<\/li>\n<li>Blood administration<\/li>\n<li>Patient blood management<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"2_Urinalysis_and_Body_Fluids_(5%2510%25)\" class=\"m-toc-anchor\"><\/span>2. Urinalysis and Body Fluids (5%-10%)<\/h3>\n<p>These questions assess your knowledge of physical and chemical testing, microscopic analysis, physiology, and disease states.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Urinalysis<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Physical<\/li>\n<li>Microscopic<\/li>\n<li>Chemical<\/li>\n<li>Renal physiology<\/li>\n<li>Disease states<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1.5em;\">II. Body Fluids<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Physical<\/li>\n<li>Microscopic<\/li>\n<li>Chemical<\/li>\n<li>Renal physiology<\/li>\n<li>Disease states<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"3_Chemistry_(17%2522%25)\" class=\"m-toc-anchor\"><\/span>3. Chemistry (17%-22%)<\/h3>\n<p>These questions assess your knowledge of carbohydrates, lipids, heme derivatives, enzymes, proteins, and other nitrogen-containing compounds. Additional topics include acid-base determinations, electrolytes, endocrinology, vitamins and nutrition, therapeutic drug monitoring, and toxicology.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. General Chemistry<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Carbohydrates<\/li>\n<li>Heme derivatives<\/li>\n<li>Lipids<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Proteins and Enzymes<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Proteins and other nitrogen-containing compounds<\/li>\n<li>Enzymes<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Acid-base, Blood Gases, and Electrolytes<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Acid-base determinations<\/li>\n<li>Electrolytes<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1.5em;\">IV. Special Chemistry<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Therapeutic drug monitoring<\/li>\n<li>Endocrinology<\/li>\n<li>Toxicology<\/li>\n<li>Vitamins and nutrition<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"4_Hematology_(17%2522%25)\" class=\"m-toc-anchor\"><\/span>4. Hematology (17%-22%)<\/h3>\n<p>These questions assess your knowledge of physiology, disease states, laboratory testing, and hemostasis (including physiology, disease states, and laboratory determinations).<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Hematology Physiology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Function<\/li>\n<li>Production<\/li>\n<li>Destruction<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Hematology Disease States<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Leukocytes (WHO classification)<\/li>\n<li>Erythrocytes<\/li>\n<li>Platelets<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Hematology Laboratory Testing<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Hemoglobin<\/li>\n<li>Cell counts<\/li>\n<li>Flow cytometry immunophenotyping<\/li>\n<li>Differentials and morphology evaluation<\/li>\n<li>Molecular and cytogenetic testing<\/li>\n<li>Hematocrit<\/li>\n<li>Indices<\/li>\n<li>Special stains<\/li>\n<li>Hemolytic indicators<\/li>\n<li>Other studies<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1.5em;\">IV. Hemostasis<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Disease states<\/li>\n<li>Physiology<\/li>\n<li>Laboratory determinations<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"5_Immunology_(5%2510%25)\" class=\"m-toc-anchor\"><\/span>5. Immunology (5%-10%)<\/h3>\n<p>These questions assess your knowledge of immunology principles, immune system diseases, transplantation, infectious disease serology, serologic procedures, and test results.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Principles of Immunology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Immunoglobulins<\/li>\n<li>Immune system physiology<\/li>\n<li>Antigen-antibody interactions<\/li>\n<li>Complement<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Diseases of the Immune System<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Hypersensitivity<\/li>\n<li>Immunodeficiency<\/li>\n<li>Autoimmunity<\/li>\n<li>Immunoproliferative diseases<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Transplantation<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>HLA typing<\/li>\n<li>Graft-vs-host disease<\/li>\n<li>Tumor immunology<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>IV. Infectious Disease Serology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Stages of infection of <em>Treponema pallidum<\/em> and <em>Borrelia burgdorferi<\/em><\/li>\n<li>Clinical significance and epidemiology of viral pathogens<\/li>\n<li>Tuberculosis infection<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>V. Serologic Procedures<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Rheumatoid factor<\/li>\n<li>Cytokine testing<\/li>\n<li>ANA<\/li>\n<li>Immunofluorescence<\/li>\n<li>Thyroid antibodies<\/li>\n<li>Nontreponemal syphilis testing<\/li>\n<li>Labeled immunoassays<\/li>\n<li>Treponemal syphilis testing<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1.5em;\">VI. Test Results<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Interpretation<\/li>\n<li>Confirmatory testing<\/li>\n<li>Disease state correlation<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"6_Microbiology_(17%2522%25)\" class=\"m-toc-anchor\"><\/span>6. Microbiology (17%-22%)<\/h3>\n<p>These questions assess your knowledge of pre-analytic procedures, analytic procedures for bacteriology, and analytic procedures for mycobacteriology, virology, parasitology, and mycology, and post-analytic procedures.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Preanalytic Procedures<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Specimen collection and transport<\/li>\n<li>Specimen processing<\/li>\n<li>Stain procedures, principles, and interpretations<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Analytic Procedures for Bacteriology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Cerebrospinal fluid<\/li>\n<li>Upper respiratory<\/li>\n<li>Lower respiratory<\/li>\n<li>Blood and bone marrow<\/li>\n<li>Body fluids from normally sterile sites<\/li>\n<li>Genital tract<\/li>\n<li>Antimicrobial susceptibility testing and antibiotic resistance<\/li>\n<li>Gastrointestinal<\/li>\n<li>MRSA\/MSSA, VRE, ESBL\/CRE screening<\/li>\n<li>Skin, soft tissue, and bone<\/li>\n<li>Urine<\/li>\n<li>BSL-3 pathogens and select agents (bioterrorism)<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Analytic Procedures for Mycobacteriology, Virology, Parasitology, and Mycology<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Mycobacteriology and <em>Nocardia<\/em> spp.<\/li>\n<li>Virology<\/li>\n<li>Parasitology<\/li>\n<li>Mycology<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary style=\"margin-bottom: 1.5em;\">IV. Postanalytic Procedures<\/summary>\n<ul style=\"list-style-type: circle; margin-top: -0.5em;\">\n<li>Documentation practices<\/li>\n<li>Critical and urgent value reporting<\/li>\n<li>Result review and autoverification<\/li>\n<li>Issuing corrected reports<\/li>\n<li>Reporting to infection control\/prevention and public health<\/li>\n<\/ul>\n<\/details>\n<h3><span id=\"7_Laboratory_Operations_(5%2510%25)\" class=\"m-toc-anchor\"><\/span>7. Laboratory Operations (5%-10%)<\/h3>\n<p>These questions assess your knowledge of quality assessments\/troubleshooting, safety, laboratory mathematics, manual and automated methodology and instrumentation, basic management principles, and education principles.<\/p>\n<details class=\"togglelist\">\n<summary style=\"margin-top: -0.5em;\">I. Quality Assessment\/Troubleshooting<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Preanalytical, analytical, and postanalytical<\/li>\n<li>Point-of-care testing<\/li>\n<li>Regulation<\/li>\n<li>Quality control<\/li>\n<li>Compliance<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>II. Safety<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Safety programs and practices<\/li>\n<li>Emergency procedures<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>III. Laboratory Mathematics<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Standard curves<\/li>\n<li>Sensitivity, specificity, and predictive value<\/li>\n<li>Molarity and normality<\/li>\n<li>Mean, median, mode and confidence intervals<\/li>\n<li>Concentration, volume, and dilution<\/li>\n<\/ul>\n<\/details>\n<details class=\"togglelist\">\n<summary>IV. Manual and Automated Methodology and Instrumentation<\/summary>\n<ul style=\"list-style-type: circle; margin-top: 0.5em;\">\n<li>Basic laboratory equipment<\/li>\n<li>Osmometry<\/li>\n<li>Nephelometry<\/li>\n<li>Electrophoresis<\/li>\n<li>Flow cytometry<\/li>\n<li>Spectrophotometry and photometry<\/li>\n<li>Chromatography<\/li>\n<li>Fluorometry<\/li>\n<li>Mass spectrometry<\/li>\n<li>Electrochemistry<\/li>\n<li>Molecular methods<\/li>\n<li>Hematology instrumentation<\/li>\n<li>Automated microbiology processors<\/li>\n<\/ul>\n<\/details>\n<div style=\"padding-left: 47px; line-height: 33px; margin-top: -0.25em;\">V. Basic Management Principles<br \/>\nVI. Education Principles<\/p>\n<\/div>\n<p><a href=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2022\/12\/MLS-Exam-Outline-01.png\"><img decoding=\"async\" class=\"aligncenter size-full wp-image-158201\" role=\"img\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2022\/12\/MLS-Outline-01.svg\" alt=\"exam outline for the ASCP MLS exam outline, which contains 100 multiple-choice questions and has a time limit of 2.5 hours\" width=\"403.7\" height=\"478.5\" \/><\/a><\/p>\n\n\t\t\t<div class=\"sg-box\">\n\t\t\t\t<div class=\"sg-box-text\">\n\t\t\t\t\t<p class=\"sg-box-title\">Check Out Mometrix's MLS Study Guide<\/p>\n\t\t\t\t\t<p>Get practice questions, video tutorials, and detailed study lessons<\/p>\n\t\t\t\t\t<a class=\"sg-box-button\" href=\"https:\/\/www.mometrix.com\/studyguides\/mls\/\" target=\"_blank\" rel=\"noopener\">Get Your Study Guide<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t<a href=\"https:\/\/www.mometrix.com\/studyguides\/mls\/\">\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"184\" height=\"238\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2025\/12\/mls.jpg\" alt=\" Study Guide\">\n\t\t\t\t<\/a>\n\t\t\t<\/div>\n<h2 class=\"pt-page\"><span id=\"How_to_Pass\" class=\"m-toc-anchor\"><\/span>How to Pass<\/h2>\n<p>Think you aren\u2019t a good test-taker? Maybe on a study-time crunch? Or just don\u2019t know how to begin studying? Mometrix has designed a new Study Secrets course to help every student, no matter what study scenario you are in. Here\u2019s what you\u2019ll find in the Study Secrets Course:<\/p>\n<ul>\n<li>Techniques to Conquer Procrastination<\/li>\n<li>Steps to building a Study Plan custom to your learning style<\/li>\n<li>7 Effective Note-Taking Methods<\/li>\n<li>Test-Taking Tips<\/li>\n<li>Memory Techniques and Mnemonics<\/li>\n<li>And much more!<\/li>\n<\/ul>\n<p>Everyone learns differently, so we\u2019ve tailored our Study Secrets Course to ensure every learner has what they need to prepare for their upcoming exam or semester. Click below to check it out!<\/p>\n<div class=\"buttonlinks\"><a href=\"https:\/\/www.mometrix.com\/university\/studysecrets\/\" class=\"class_names\" style=\"color:black;\" onclick=\"_paq.push(['trackEvent', 'Course Button', 'Course Click', Study Course Click']);\">Study Secrets Online Course<\/a><\/div>\n<h2 class=\"pt-page\"><span id=\"Registration\" class=\"m-toc-anchor\"><\/span>Registration<\/h2>\n<p>To apply for the exam, you will need to create an ASCP account on their website and fill out the application packet. Once you have submitted the application and the $250 application fee, you will need to then upload any supporting documentation that may be required via the eligibility route you have chosen. ASCP will not notify you of missing documents or discrepancies.<\/p>\n<p>When your application is approved, you will receive an Admission Notice email. When you receive the email, log on to your ASCP account to view your Admission Notice. The notice includes contact information for Pearson VUE to schedule your exam and your exam eligibility period. The exam is conducted at Pearson VUE Test Centers located nationwide and internationally. Most test centers offer appointments Monday through Saturday, holidays excepted.<\/p>\n<h2 class=\"pt-page\"><span id=\"Test_Day\" class=\"m-toc-anchor\"><\/span>Test Day<\/h2>\n<p>On your test day, you must arrive at the testing center at least 30 minutes before your appointment. Once you arrive, you will be asked to show a form of valid, government-issued photo ID. The facilitator will then inspect your identification, collect your signature and biometric information (using biometric technology), and assign a locker for you to place your belongings in during the exam.<\/p>\n<p>You may have a non-programmable calculator with you at your testing center. Everything else, including your cell phone and keys, must be secured in your assigned locker. Scratch paper or a dry-erase board will be provided for notes.<\/p>\n<h2 class=\"pt-page\"><span id=\"Test_Scores\" class=\"m-toc-anchor\"><\/span>Test Scores<\/h2>\n<p>As mentioned previously, the MLS exam is computer-adaptive. Here\u2019s a look at how it works:<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-138529\" role=\"img\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2022\/08\/Computer-Adaptive-Flowchart.svg\" alt=\"A flowchart that shows the adaptive nature of the exam\" width=\"467\" height=\"746\" \/><br \/>\n<img decoding=\"async\" class=\"desktopbuttons\" style=\"float: left; margin-right: 2em; margin-top: 1em;\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2022\/09\/Difficulty-Ratings-for-Histotechnician-HT-Exam-Questions.svg\" alt=\"\" width=\"188.51\" height=\"449.26\" \/><\/p>\n<div>\n<p>The first question is judged to be of medium difficulty, and depending on your performance, the next question may be easier or harder. If you do well in the first question, the second question will be harder; conversely, if you do poorly on the first question, the second question will be easy.<\/p>\n<p>The questions on the MLS exam are rated on a scale of 100-999. The higher a question is rated, the harder the question is (e.g., a question marked as 566 will be more difficult than a question marked 481).<\/p>\n<p><strong>To pass the exam, your final question must be on or above the 400 mark.<\/strong> If your final question is below this mark, you will not pass the exam, even if you have answered some questions above the 400 mark at some point during the exam. This is because the computer has rated the difficulty of your final question based on how you answered the previous questions.<\/p>\n<\/div>\n<h3><span id=\"Receiving_Your_Scores\" class=\"m-toc-anchor\"><\/span>Receiving Your Scores<\/h3>\n<p>Within four business days after taking your exam, you will receive an email notifying you that your results are available in your account. If you need to send your results to a third party, a verification request link is listed on your score report with instructions.<\/p>\n\n\t\t\t<div class=\"sg-box\">\n\t\t\t\t<div class=\"sg-box-text\">\n\t\t\t\t\t<p class=\"sg-box-title\">Check Out Mometrix's MLS Flashcards<\/p>\n\t\t\t\t\t<p>Get complex  subjects broken down into easily understandable concepts<\/p>\n\t\t\t\t\t<a class=\"sg-box-button blue-button\" href=\"https:\/\/www.flashcardsecrets.com\/mls\/\" target=\"_blank\" rel=\"noopener\">Get Your Flashcards<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t<a href=\"https:\/\/www.flashcardsecrets.com\/mls\/\">\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"238\" height=\"184\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2025\/12\/mlsflash.jpg\" alt=\" Flashcards\">\n\t\t\t\t<\/a>\n\t\t\t<\/div>\n<h2 class=\"pt-page\"><span id=\"FAQs\" class=\"m-toc-anchor\"><\/span>FAQs<\/h2>\n<div class=\"faq-list\">\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n<h4 class=\"letter\">Q<\/h4>\n<p style=\"line-height: unset;\">How many questions are on the ASCP MLS exam?<\/p>\n<\/p><\/div>\n<div class=\"a_item\">\n<h4 class=\"letter text_bold\">A<\/h4>\n<p>There are 100 multiple-choice questions<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n<h4 class=\"letter\">Q<\/h4>\n<p style=\"line-height: unset;\">How long is the ASCP MLS exam?<\/p>\n<\/p><\/div>\n<div class=\"a_item\">\n<h4 class=\"letter text_bold\">A<\/h4>\n<p>The time limit for the exam is 2.5 hours.<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n<h4 class=\"letter\">Q<\/h4>\n<p style=\"line-height: unset;\">What is the passing score for the ASCP MLS exam?<\/p>\n<\/p><\/div>\n<div class=\"a_item\">\n<h4 class=\"letter text_bold\">A<\/h4>\n<p>To pass the exam, you will need a minimum scaled score of 400.<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<div class=\"qa_wrap\">\n<div class=\"q_item text_bold\">\n<h4 class=\"letter\">Q<\/h4>\n<p style=\"line-height: unset;\">How much does the ASCP MLS exam cost?<\/p>\n<\/p><\/div>\n<div class=\"a_item\">\n<h4 class=\"letter text_bold\">A<\/h4>\n<p>The application fee for the exam is $250.<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n\n<p><div itemscope=\"\" itemprop=\"author\" itemtype=\"http:\/\/schema.org\/Person\" class=\"author-bio\"><img loading=\"lazy\" decoding=\"async\" itemprop=\"image\" class=\"author-image\" height=\"127\" width=\"127\" alt=\"Headshot of Mometrix associate Peter Rench.\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2024\/01\/Peter-Rench-scaled.webp\"><div class=\"author-text\"><p class=\"author-name\">By <span itemprop=\"name\">Peter Rench<\/span><\/p><div class=\"author-desc\"><p>Peter Rench joined Mometrix in 2009 and serves as Vice President of Product Development, responsible for overseeing all new product development and quality improvements. Mr. Rench, a National Merit Scholar, graduated magna cum laude with a Bachelor of Science in Mechanical Engineering and a minor in mathematics from Texas A&amp;M University.<\/p>\n<\/div><\/div><\/div><div itemscope=\"\" itemprop=\"editor\" itemtype=\"http:\/\/schema.org\/Person\" class=\"author-bio\"><img loading=\"lazy\" decoding=\"async\" itemprop=\"image\" class=\"author-image\" height=\"127\" width=\"127\" alt=\"Aaron Lanni profile pic\" src=\"https:\/\/www.mometrix.com\/academy\/wp-content\/uploads\/2024\/12\/image_2024-12-11_124906662.png\"><div class=\"author-text\"><p class=\"author-name\">Edited by <span itemprop=\"name\">Aaron Lanni<\/span><\/p><div class=\"author-desc\"><p>Aaron is the content manager and lead editor for Mometrix Academy. He regularly produces, updates, proofreads, and edits content to ensure it meets Mometrix\u2019s quality and accessibility standards.<\/p>\n<\/div><p class=\"alignright author-desc author-link\"><a class=\"ylist\" href=\"https:\/\/www.mometrix.com\/academy\/editorial-guidelines\/\">Learn more about our editorial guidelines<\/a> \u2192<\/p><\/div><\/div><\/p>\n<p class=\"disclaimer\">Mometrix Test Preparation is not affiliated with or endorsed by any official testing organization. All organizational and test names are trademarks of their respective owners.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The American Society for Clinical Pathology (ASCP) administers the Medical Laboratory Science (MLS) exam for those wishing to obtain a credential as a medical laboratory scientist. Click \u201cStart Test\u201d above to take a free ASCP Medical Laboratory Science practice test, and check out our premium-quality ASCP Medical Laboratory Science test prep resources by clicking the &#8230; <a title=\"ASCP Medical Laboratory Science Exam\" class=\"read-more\" href=\"https:\/\/www.mometrix.com\/academy\/medical-laboratory-science-practice-test\/\" aria-label=\"Read more about ASCP Medical Laboratory Science Exam\">Read more<\/a><\/p>\n","protected":false},"author":19,"featured_media":206723,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":{"0":"post-158126","1":"page","2":"type-page","3":"status-publish","4":"has-post-thumbnail","6":"page_type-exam-overview","7":"page_domain-ascp-mls"},"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/pages\/158126","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/comments?post=158126"}],"version-history":[{"count":6,"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/pages\/158126\/revisions"}],"predecessor-version":[{"id":294452,"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/pages\/158126\/revisions\/294452"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/media\/206723"}],"wp:attachment":[{"href":"https:\/\/www.mometrix.com\/academy\/wp-json\/wp\/v2\/media?parent=158126"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}